Ukrainian Journal of Forest and Wood Science

The method of producing aseptic viable Salix matsudana Koidz. 'Tortuosa' Rehd. explants isolated from donors in different phenophases was developed and The optimal conditions for callus formation in explants tissue with a frequency of more than 90% were studied. The biotechnology of micropropagation, which included the selection of the components of culture media for various stages and types of morphogenesis, was developed and it allowed obtaining a significant number of regenerants in a short time for further use in gardening.

salix matsudana Koidz. 'Tortuosa' Rehd., culture in vitro, explants, micropropagation, callus tissue, basal medium, plant-regenerant